# Concept and theory¶

## Summary¶

The computation of the compliance \(J\) for dielectric, elastic, spheroidal objects in the OS can be divided into three main tasks: measuring the deformation \(w\), modeling the optical stress \(\sigma_r\), and computing the GGF from the stress. Several approaches to these problems have been presented in the related literature and are discussed in the following.

## Experimentally quantifying deformation¶

The deformation is quantified from video images by fitting an ellipse to the contour of the stretched object. The deformation can then be defined as a relation between the semimajor or semiminor axes and the initial radius (or semiaxes). Note that the prolate spheroidal shape is only an approximation to the actual shape. The methods in this package more or less assume that this approximation is valid.

## Optical stress profile acting on a prolate spheroid¶

The optical stress \(\sigma(\theta)\) in dependence of the angle \(\theta\) is a result of the optical forces acting on the surface of the spheroid. The angle \(\theta\) is defined in the imaging plane in a typical OS experiment, with \(\theta=0\) pointing to the right hand fiber.

### \(\cos^2\theta\) approximation¶

Ray optics is used to compute the optical stress acting on a spheroid and a \(\sigma_0 cos^2\theta\) model is fitted to the resulting stress profile with the peak stress \(\sigma_0\) [Guck2001]. The \(\sigma_0 cos^2\theta\) approximation simplifies subsequent computations.

Note that a more general model \(\sigma_0 cos^2n\theta\) with larger exponents (e.g. \(n\) = 2,3,4,…) can also be applied, e.g. for different fibroblast cell lines [Ananthakrishnan2006].

### Semi-analytical perturbation approach (Boyde et al. 2009)¶

- gaussian laser beam
- \(a > \lambda\): higher order perturbation theory
- [Boyde2009]

### Generalized Lorentz-Mie theory (Boyde et al. 2012)¶

- gaussian laser beam
- spheroidal coordinates
- generalized Lorenz–Mie theory
- not implemented (Matlab sources available upon request)
- [Boyde2012]

## Computation of the GGF¶

The following derivations are based on the theoretical considerations of Lur’e [Lure1964] for a rotationally symmetric deformation of a sphere (which in general does not result in prolate spheroids) and their application to the OS by Ananthakrishnan et al. [Ananthakrishnan2006]. Note that a corrigendum has been published for this article in 2008 [Ananthakrishnan2008Corr].

### General approach¶

The GGF connects the measured deformation to the shear modulus \(G\) which, in OS literature, is usually written in the form

where \(w\) is the change in radius of the stretched sphere along the stretcher axis and \(r_0\) is the radius of the unstretched sphere. Note that the quantity \(w/r_0\) resembles a measure of strain along the stretcher axis.

The GGF can be computed from the radial stress \(\sigma_r(\theta)\) via the radial displacement \(u_r(r, \theta)\). These quantities can be connected via a Legendre decomposition according to ([Lure1964], chapter 6)

with the Legendre polynomials \(P_n\) and the Poisson’s ratio \(\nu\). The coefficients \(A_n\) and \(B_n\) have to be determined from boundary conditions. For the case of normal loading, which is given by the electromagnetic boundary conditions in the OS (\(\sigma_\theta=\tau_{r,\theta}=0\)), these coefficients compute to:

and for \(n>=2\):

Where \(s_n\) is the \(n\text{th}\) component of the Legendre decomposition of \(\sigma_r\)

The radial displacement then takes the form

with the coefficients \(L_n\) and \(M_n\) given in [Lure1964], chapter 6.6. We measure the displacement at the outer perimeter of the stretched sphere and on the stretcher axis only; Thus, we set \(r=r_0\) and \(\theta=0\) with \(w=u_r(r_0, 0)\).

To obtain the GGF, we finally compute

Notes:

- Due to the fact hat the stress profile in the OS is rotationally symmetric w.r.t. the stretcher axis, all odd coefficients \(s_n\) are zero.
- The polar displacement \(u_\theta\) has been omitted here, because it does not represent a quantity measurable in an OS experiment.

### Special case: \(\cos^2\theta\) approximation¶

Following the above approach, the stress profile

with the peak stress \(\sigma_0\) can be decomposed into two Legendre polynomials

Inserting these Legendre coefficients in the above equation for the GGF yields

Historically, the relation between strain, stress, and shear modulus was written in the form

with the geometrical factor \(F_\text{G}\) that does not include the peak stress \(\sigma_0\). Hence the term “global geometrical factor” \(\text{GGF} = \sigma_0 F_\text{G}\).

## Computation of compliance¶

A typical OS experiment records the deformation \(w(t)\) over time \(t\). The quantity of interest is the (creep) compliance \(J(t)\). With \(J = 1/G\), it computes to

Note that the GGF is now time-dependent, because the optical stress profile \(\sigma_r\), from which the GGF is computed, also depends on the deformation.